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SummaryOnly aggregates of hordeum mosaic virus were encountered in cytoplasm of cells fixed in a fixative containing chromate (pH 7.2) and post-fixed in osmic acid. In contrast, hordeum mosaic virus inclusions in wheat leaf cells appeared after glutaraldehyde and osmic acid fixation as aggregates in well-fixed dense cytoplasm, as partly disrupted inclusions, or as loosely occurring virus in incompletely fixed cytoplasm. The results showed that use of more than one fixative in an investigation of plant virus infected tissue is useful for extending and interpreting ultrastructural observations.

The Appearance of Hordeum Mosaic Virus in Wheat Leaf Cells after Fixation in Glutaraldehyde or Chromate