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SummaryThe infectious agent in culture fluids of serially passed nuclear polyhedrosis virus of the cabbage looper (Trichoplusia ni) sediments at 830S. Infectivity falls below detectable limits when virus concentrates are treated with either deoxycholate (DOC) or with Tween-ether, and the sedimentation coefficient becomes 325S. The buoyant density in CsCl of several virus preparations was in the range 1.26 to 1.35 g/ml and increased to 1.42 in all cases after DOC treatment. The results indicate that the non-occluded form of the virus produced in tissue culture consists of fragile enveloped particles.

Some Biophysical Properties of Virus Present in Tissue Cultures Infected with the Nuclear Polyhedrosis Virus of Trichoplusia ni