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SUMMARY Inactivation of Japanese encephalitis virus by Na deoxycholate was due to release of RNA-like material sensitive to RNase and detectable under assay conditions for infectious RNA. The RNA-like material released by Na deoxycholate was similar to infectious RNA extracted by phenol in such properties as RNase sensitivity, increased plaque formation in hypertonic salt and ether resistance, but differed from it in such characteristics as heat- lability and sensitivity to isobutanol. The mechanism of inactivation of the virus by ether was not quite identical with that by Na deoxycholate treat- ment, because ether treatment might have denatured virus protein and damaged the RNA.

A Comparison of the Inactivation of Japanese Encephalitis Virus by Deoxycholate and Ether