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SummaryThe ionic strength and ionic composition of the medium can markedly affect certain aspects of virus replication or maturation in the case of some picornaviruses (Wallis & Melnick, 1962; Tolskoya et al. 1966; Fiala & Kenny, 1967; Blough et al. 1969; Agol et al. 1970) and in the case of Sindbis virus (SV), a group A togavirus (Waite & Pfefferkorn, 1970). In general, a lowered ionic strength appears to inhibit while a higher ionic strength increases virus yield. Blough et al. also reported enhanced crystallization of rhinovirus in HeLa cells after increasing the concentration of MgCl2 in the medium. Our observations described below show that the yield of dengue virus (DV), a group B togavirus, is also affected by the ionic strength of the medium. However, no clear-cut effect on the crystallization of DV in Vero cells (Matsumura, Stollar & Schlesinger, 1971) was noted after increasing the MgCl2 concentration.

Effects of Ionic Strength on the Release of Dengue Virus from Vero Cells