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SUMMARY The mol. wt. of the single-stranded RNA molecules synthesized in a cell-free system on simian virus 4o (SV4 o) DNA by Escherichia coli RNA polymerase was determined at intervals after the initiation of the reaction. Chain elongation of the RNA, under the experimental conditions used, took place at an average rate of 5 nucleotides/s. Thus single-stranded RNA molecules of I "5 × 106 mol. wt. resulting from continuous transcription of one turn of the circular SV4o genome did not appear until about 2o min after initiation of the reaction. After 4 ° to 8o min, molecules with mol. wt. equal to or greater than the mol. wt. of the DNA template strand (/> I"5 x lO 6) accounted for 25 to 5o% of the mass of the RNA population. Up to 25 % of the RNA synthesized at that time had mol. wt. equal to or larger than 3 × lO6. The results indicate that the cell-free system used does not contain specific factors or signals which would cause the E. coli RNA polymerase to terminate transcription.

journal of general virology

Synthesis in vitro by Bacterial RNA Polymerase of Simian Virus 40-specific RNA: Multiple Transcription of the DNA Template into a Continuous Polyribonucleotide