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Brome mosaic virus (BMV) and cowpea chlorotic mottle virus (CCMV) are spherical plant viruses which have recently been shown to possess divided genomes (Lane & Kaesberg, 1971; Bancroft, 1971) even though the virus particles sediment as single species. BMV contains four species of RNA with molecular weights of 1.09 × 106, 0.99 × 106, 0.75 × 106 and 0.28 × 106 and CCMV contains four species with molecular weights of 1.15, 1.0, 0.85 and 0.32 × 106 (Fowlks & Young, 1970). The species are named 1, 2, 3 and 4, respectively. Species 1 + 2 + 3 are required to initiate BMV infections (Lane & Kaesberg, 1971) but CCMV seems only to require species 1 + 2, although the addition of species 3 enhances lesion production (Bancroft, 1971). Species 4 is needed by neither virus for infection. I wished to determine if a virus possessing some properties of both viruses could be obtained by mixing BMV species 1 + 2 with CCMV species 3 prior to biological assay.

A Virus Made from Parts of the Genomes of Brome Mosaic and Cowpea Chlorotic Mottle Viruses