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SUMMARY The inactivation of Semliki Forest virus in solutions of guanidine hydrochloride and urea shows an anomalous dependence on concentration. Under some circum- stances the inactivation is reversible. This reactivation is non-cooperative and extra- cellular and can be induced by incubation at high, or at negligible inactivant con- centrations. It is suggested that changes in the surface structure of the virus particle take place before or during inactivation and that modifications of the conformation of the virus are involved in the reversible reactions.

Inactivation and Reactivation of Semliki Forest Virus by Urea and Guanidine Hydrochloride