English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

The defective genome of murine sarcoma virus (MSV)*(moloney isolate) may be rescued from non-producer hamster tumour cells (HT-1) by co-cultivation of the latter and mouse embryo cells with added helper murine leukaemia viruses (MuLV) (Huebner et al. 1966; Huebner, 1967). Rescue of MSV from HT-1 cells by co-cultivation with MuLV-carrier cells has not, however, been described. This report describes the retrieval of MSV from mixed cultures of HT-1 and various MuLV-carrier cells of mouse and hamster origin. Moreover, it was found that the frequency of success and titres of MSV recovered by co-cultivation of HT-1 and F4, one of the MuLV-carrier mouse cell lines, were much higher than those obtained by the conventional method. We have analysed the factors responsible for such high efficiency of genome rescue.Among the MuLV-carrier cell lines used, F4 and F5 were derived from two of nine Rauscher pseudotype MSV (MSV(RLV) transformed foci of NIH Swiss mouse embryo cells isolated by trypsinization within a cloning ring.

Rescue of Murine Sarcoma Virus Genome in Mixed Cultures of 'Non-producer' Hamster Tumour Cells and Helper Virus Carrier Cell Lines