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Alcaligenes aquamarinus and Alcaligenes faecalis subsp. homari proved to be identical to Deleya aesta in respiratory quinone type, cellular fatty acid profiles, and biochemical and physiological characteristics and as determined by deoxyribonucleic acid-deoxyribonucleic acid relatedness studies. The name Akaligenes aqua- marinus has priority. Therefore, the name Deleya aquamarina comb. nov. is proposed for the type species of the genus Deleya; the type strain should be changed from strain IAM 12551 (= ATCC 27128) (type strain of Deleya aesta) to strain IAM 12550 (= ATCC 14400) (type strain of Alcaligenes aquamarinus). A study of the relationships of marine and other species of the genera Alcaligenes and Deleya was initiated to deter- mine the correct taxonomic placement of the marine species. Of particular interest in this group were Alcaligenes aqua- marinus (ZoBell and Upham 1944) Hendrie, Holding, and Shewan 1974 (Approved Lists), Alcaligenes faecalis subsp. homari Austin, Rodgers, Forns, and Colwell 1981, and Deleya aesta (Baumann, Baumann, Mandel, and Allen 1972) Baumann, Bowditch, and Baumann 1983, which is the type species of the genus Deleya Baumann, Bowditch, and Baumann 1983. As a result of chemotaxonomic studies (Miyazaki et al., manuscript in preparation), all of the marine strains of the genus Alcaligenes can be expected to be classified in the genus Deleya. MATERIALS AND METHODS The bacterial strains which we studied are listed in Table 1. Cultures were preserved by freeze-drying. The suspension fluid used for freeze-drying was a 1: 1 mixture of horse serum and a sucrose-salts aqueous solution containing 20% (wthol) sucrose, 0.4% (wthol) NaCl, and 0.2% (wtlvol) MgCl,. Cultures were maintained on slopes of modified marine agar (MMA) at 5°C by transfer every month. MMA was com- posed of 5.0 g of peptone (Kyokuto), 1.0 g of yeast extract (Oriental), 15.0 g of Bacto-Agar (catalog no. 0410; Difco Laboratories, Detroit, Mich.) and 1,000 ml of 0.75~ seawa- ter (75% seawater in distilled water) adjusted to pH 7.4 before autoclaving. For the moderately halophilic strain of Deleya halophila, NaCl was added to a final concentration of 10% (wthol). The Gram stain reaction was determined by using smears from cultures grown for 1 day on MMA plates at 25°C. Flagellar arrangement was determined by transmission elec- tron microscopy of cultures that were grown on MMA for 1 or 2 days at 25°C and suspended in 2 or 3 ml of a 3% NaCl solution. Cell suspensions were placed on Formvar-coated grids, fixed, and negatively stained with 1% phosphotungstic acid adjusted to pH 7.0 by using a 1 N NaOH solution. Immediately after blot drying, the grid was observed by using a model JEOL 200CX electron microscope at 100 kV. Production of acid from glucose and mannitol under aerobic and anaerobic conditions was determined in medium composed of 1.0 g of Casitone (catalog no. 0259; Difco), 0.1 g of yeast extract (catalog no. 0127; Difco), 0.5 g of (NH,),SO,, 0.5 g of tris(hydroxymethy1) aminomethane, 10.0 g of carbohydrate, and 1,000 ml of 0.5~ seawater adjusted to pH 7.4. Carbohydrates were autoclaved sepa- rately. The cultures were incubated for 3 days. GasPak anaerobic systems (BBL Microbiology Systems, Cockeys- ville, Md.) were used for anaerobic incubation. Growth was observed visually, and acid production was confirmed by adding cresol red to aerobic cultures and bromothymol blue to anaerobic cultures. The inocula for tests were prepared from cultures that were grown on MMA slopes or in modi-

international journal of systematic bacteriology

Synonymy of Alcaligenes aquamarinus, Alcaligenes faecalis subsp. homari, and Deleya aesta: Deleya aquamarina comb. nov. as the Type Species of the Genus Deleya