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Among the mycoplasmal species commonly isolated from porcine lungs are Mycoplasma hyopneumoniae and Mycoplasma flocculare. The former organism is a proven agent of enzootic porcine pneumonia, whereas the latter organism is of little or no pathogenicity. These mycoplasmas show considerable antigenic cross-reactivity. The deoxyribonucleic acids of M. hyopneumoniae strain 11 and M. flocculare strain Ms42T (T = type strain) each had a guanine-plus-cytosine content of 33 mol%. We also determined the extent of deoxyribonucleic acid sequence homology as reflected in deoxyribonucleic acid-deoxyribonucleic acid hybridization values. Approximately 10% heterologous hybridization was observed. Thus, our data support the previous classification of these organisms as separate species within the genus Mycoplasma.

Guanine-Plus-Cytosine Compositions of and Deoxyribonucleic Acid Hybridization Comparisons between Mycoplasma hyopneumoniae and Mycoplasma flocculare