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The cellular proteins of eight strains of Actinobacillus actinomycetemcomitans and seven strains of Haemophilus aphrophilus were analyzed by two-dimensional electrophoresis and silver staining. The electrophoretic patterns of A. actinomycetemcomitans and H. aphrophilus were so different that distinction between these closely related bacteria could easily be made. There were also minor quantitative and qualitative differences in the protein patterns of strains within both species, enabling, particularly, A. actinomycetemcomitans strains FDC 2097 and FDC Y4 and H. aphrophilus strains ATCC 19415, FDC 626, and FDC 621 to be singled out.

international journal of systematic bacteriology

Notes: Differentiation Between Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus by High-Resolution, Two-Dimensional Protein Electrophoresis