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The current descriptions of the genus Leptospira Noguchi and the species L. interrogans and L. bifexa (Int. J. Syst. Bacteriol. 30:225-420) are inadequate and out of date. On behalf of the members of the Subcommittee on the Taxonomy of Leptospiru of the International Committee on Systematic Bacteriology, amended descriptions of these taxa are presented. Definition of the genus Leptospira Noguchi 1917,755 (4). (Lep.to.spi'ra. Gr. adj. leptos thin, narrow, fine; Gr. n. spira a coil; M.L. fem.n. Leptospiru a fine coil.) Flexible, helicoidal orga- nisms which are usually 6 to 20 pm long and about 0.1 pm in diameter. The coils have an amplitude of about 0.1 to 0.15 pm and a wave- length (pitch) of about 0.5 pm. In liquid milieux, one or both ends are usually hooked or bent, but either one or both ends may become straight- ened temporarily. In some cultures the majority or all of the cells regularly have straight ends which have not been observed to become hooked. The organism is not visible by ordinary bright-field microscopy, but it is observed clear- ly by dark-field illumination and phase-contrast microscopy. The cells are not easily visualized after staining by aniline dyes, but they can be demonstrated by silver deposition techniques and by other special staining methods (e.g.,

international journal of systematic bacteriology

Amended Descriptions of the Genus Leptospira Noguchi 1917 and the Species L. interrogans (Stimson 1907) Wenyon 1926 and L. biflexa (Wolbach and Binger 1914) Noguchi 1918