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Analysis of representative strains of the eight human serotypes of Ureaplasma urealyticum by polyacrylamide gel electrophoresis identified 36 to 40 polypeptides for each strain. At least 80% of the peptides were common among strains, but unique major peptides were identifiable in ureaplasmic types. Type 1 had a polypeptide of 85,000 daltons, type 3 had a polypeptide of 72,000 daltons, type 5 had a polypeptide of 64,000 daltons, and type 8 had a polypeptide of 95,000 daltons. The unique polypeptides in types 1 and 8 were identified as membrane components. Two common major components of 44,000 and 70,000 daltons were observed. Several components were common to some, but not all, serotypes. Patterns obtained from U. urealyticum strains were strikingly different from the patterns of Acholeplasma laidlawii, Mycoplasma gallisepticum, Mycoplasma arginini, and Mycoplama hominis. Isoelectric focusing demonstrated a unique membrane protein for type 1 at pK 6.4, whereas type 8 possessed an assembly of five unique proteins at pK 7.0. Ureaplasmata were strongly similar to each other by isoelectric focusing, but strikingly different from members of the other genera studied. Although a filtered, strongly buffered dialysate medium with 1% serum and 30 mM urea was used both to maximize yields and to minimize contamination, minor contaminants were detected, which comigrated with horse transfenin (pK 6.0) and cytochrome c (molecular weight, 14,000). The similarities of the polypep- tide patterns of U. urealyticum strains affirm their close relationships to each other, in contrast to the diversity shown in the genus Mycoplama, and our recognition of type-specific membrane peptides will enhance the identification of serotypes and the classification of strains. The "T" Mycoplasma strains, isolated from humans, have been classified as a single species, Ureaplasma urealyticum, with eight serotypes (23). The basis for differentiation of the sero- types was the fact that strains of U. urealyticum can be typed reasonably specifically by the met- abolic inhibition test or by growth inhibition on agar. However, the metabolic inhibition test shows much greater species specificity than type specificity in the differentiation of Mycoplasma species. Thus, we really do not know whether types of U. urealyticum are as similar to each other as strains of a classic Mycoplasma species or whether the relationship between types is more like that observed between Mycoplasma species which are serologically related (7). The identification of Mycoplasrna species and the determination of the relationships among strains of a species and of related species have been carried out by polyacrylamide gel electro- phoresis; patterns from related organisms are quite similar, and those from unrelated orga- nisms are markedly dissimilar (17). The purpose of the present study was to compare the poly- peptides and proteins of representative strains of the eight serotypes of U. urealyticum by examining both molecular weights (sodium do- decyl sulfate (SDS )-polyacrylamide gel electro- phoresis) and isoelectric points (isoelectric fo- cusing). Comparisons were also made with strains of Mycoplasma and Acholeplasma spe- cies, and extensive control measures for moni- toring contamination with medium components were carried out to venfy the results.

international journal of systematic bacteriology

Comparison of the Proteins and Polypeptides of the Eight Serotypes of Ureaplasma urealyticum by Isoelectric Focusing and Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis