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The degree of binding was determined between deoxyribonucleic acid (DNA) preparations from nocardiae and "rhodochrous" strains and uracil-labeled DNA from three reference strains, Nocardia asteroides N668 and rhodochrous strains N11 and N54. In all cases, good congruence was found between the DNA reassociation data and that from numerical phenetic studies. Only a small degree of nucleotide sequence homology was found between the N. asteroides reference system and the other taxa studied, and there was evidence that N. crsteroides is genetically heterogeneous. The moles percent guanine plus cyto- sine for the rhodochrous strains was within the range 58 to 67; the correspond- ing range for nocardiae was 64 to 68 mol%. The confused and tortuous taxonomic history of bacteria variously known as "Mycobacte- rium" rhodochrous, the "rhodochrous" com- plex, and the "rhodochrous" taxon was re- viewed by Bousfield and Goodfellow (51, who concluded that these organisms form a recog- nisable taxon equivalent in rank to the genera Corynebacterium, Nocardia, and Mycobacte- rium. The internal structure of the rhodochrous complex is still obscure, but the results of chem- ical (2, 3, 17), numerical phenetic (17, 39), de- oxyribonucleic acid (DNA) reassociation (lo), genetic recombination (l), and serological (24) studies show that subgroups exist. The heterogeneity of the rhodochrous com-

Classification of the "Rhodochrous" Complex and Allied Taxa Based upon Deoxyribonucleic Acid Reassociation