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The mole percent guanine plus cytosine in the deoxyribonucleic acid of 12 strains (6 species) of Leuconostoc and 9 strains (4 species) of slime-forming heterofermentative lactobacilli has been determined. Calculated from melting- temperature curves, the values were in the range 40 to 4776, but calculated from buoyant-density determinations they were 2 to 4% lower. The melting temperature of the reference deoxyribonucleic acid from Escherichia coli K-12 was 0.7 to 1 degree higher than the expected figure of 90.6 C. This point is discussed. The mole percent guanine plus cytosine (%G+C) in the deoxyribonucleic acid (DNA) of bacteria classified in the genus Leuconostoc is known only for a single strain of Leuconostoc rnesenteroides (6, 10). G+C data for representa- tive strains of all the species of Leuconostoc are reported here and are compared with those of representative strains of slim e-forming species of the het eroferment ative lactobacilli, namely, Lactobacillus confusus, Lactobacillus virides- cens, Lactobacillus vermiforme, and Lacto- bacillus tr ic h od es.

Guanine Plus Cytosine Content of the Deoxyribonucleic Acid of the Leuconostocs and Some Heterofermentative Lactobacilli