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The serological relationship between 125 strains of Nocardia and Mycobac- terium was analyzed by means of the comparative immunodiffusion technique. Two reference precipitation systems, N. corallina and "M. " pellegrino, were utilized. Most of the tested strains had one to three precipitinogens in common with the N. corallina and the "M. " pellegrino reference strains. Most of the mycobacterial and most of the nocardia strains examined seemed to be serologically related to the two reference strains to an equally large extent. Eight strains, either designated N. corallina, N. rubra, or "M. "rhodochrous, revealed a close serological relationship with the N, corallina reference strain, and so it might be justified to consider these strains as belonging to one taxonomic group. Ten other strains, designated N. rubra, "M. " pellegrino, or "M. " rhodochrous, displayed a close serological relationship with the "M. " pellegrino reference strain, and they may therefore be regarded as belonging to another taxonomic group. Eight strains with the designations N. corallina, N. rubra, or "M." rhodochrous showed little serological similarity to either of the two reference strains. Thus, the present taxonomy of a number of strains of Nocardia and Mycobacterium seems questionable since some strains were found to be serologically different, although referred to the same species, whereas some strains, referred to different species, were serologically closely related. The classification and nomenclature of such strains should, therefore, be reconsid- ered.

Serological Study of Nocardiae and Mycobacteria by Using "Mycobacterium" pellegrino and Nocardia corallina Precipitation Reference Systems