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Among the five generally recognized species in the genus Brucella Meyer and Shaw, a type strain exists only for Brucella neotomae Stoenner and Lackman. Described herein are the characteristics of B. abortus (Schmidt and Weis) Meyer and Shaw, strain 544 (ATCC 23488); B. suis Huddleson, strain 1330 (ATCC 23444); B. melitensis (Hughes) Meyer and Shaw, strain 16M (ATCC 23456); and B. ovis Buddle strain 6690 (ATCC 25840). Each of these strains has features similar to those reported for the original isolates of the species, each simultaneously fits the revised species descriptions and, when viewed in toto, these strains reflect the current concept of this genus. Thus, in accordance with the recommendations of the International Subcom- mittee on the Taxonomy of the Genus Brucellu, these strains here are designated as the neotype strains of these species. Because three of the species in this genus are divided into biotypes that are of epidemiological significance we have, again at the recommendation of the International Subcommittee, selected a representative strain for each biotype for use as a reference. The characteristics of each of the reference strains are also described herein. At its meeting in Mexico City in 1970, the Subcommittee on the Genus Brucella of the Inter- national Committee on Nomenclature of Bacteria recommended the recognition of five species in this genus, viz., Brucella abortus, B. melitensis, B. neotomae, B. ovis, and B. suis. The Subcommittee also recommended that neotype strains be pro- posed for each of the species that lacks an extant type strain and that reference strains of each biotype within the species be designated and submitted for deposit with the American Type Culture Collection. The purpose of this paper is to designate and describe these neotype and reference strains.

Designation of Neotype Strains and of Biotype Reference Strains for Species of the Genus Brucella Meyer and Shaw