Studies in the Biochemistry of Micro-organisms Part III.- Quantitative examination by the carbon balance sheet method of the types of products formed from glucose by species of Aspergillus

The first large group of fungi chosen for investigation by the methods described in Part II was the Aspergillus group. The Aspergilli were chosen primarily because they are relatively easy to grow on synthetic media and because a wide variety of cultures was available in different parts of the world. Throughout this work conditions have been kept as nearly standard as possible so that results obtained at any time during the progress of the work should be comparable. With this end in view all the metabolism experiments were carried out at one temperature, namely, 23-25° C. One medium, consisting of a Czapek-Dox medium of the composition given in Part I, p. 7, was used throughout. This medium was made up in large amounts and pipetted out accurately, in 250 c.c. quantities, into a number of 750 c.c. conical flasks.