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In a communication published in the “Proceedings of the Royal Society” (vol. xxviii, p. 260), I drew attention to the employment of an ammoniated cupric liquid for the volumetric estimation of sugar. Instead of giving an altogether new formula for this liquid, I recommended it to be prepared from Fehling’s solution with the addition of a certain volume of ammonia and of water. Under the method of analytical procedure adopted in my laboratory, which consisted in running in the liquid pretty quickly from the burette to near the required point, and then dropping it in slowly, the amount of soda contained in Fehling’s solution proved sufficient to give accurate results.

XXVIII. Supplementary note on the volumetric estimation of sugar by an ammoniated cupric test giving reduction without precipitation.