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The main object of the investigations, the results of which are embodied in this paper, was to repeat certain experiments performed by Tornier (1908) and to confirm or confute his conclusions. Tornier experimented with the eggs of Amphibia—Rana, Bufo and in particular the Axolotl (Amblystoma ), keeping them for 48 hours in an 8 per cent. solution of cane sugar, then transferring them for 24 hours to a 2 per cent. Solution, and after that to well aerated water in which they completed their development. As a result of this treatment, in further development all sorts of abnormalities, turned up, such as short bodies, swollen abdomens, enlarged mouth cavities, etc. Since similar abnormalities were produced by the use of solutions of glycerine and common salt, Tornier concluded that the energy of the germinal part of the egg had been affected by the reagents used, and that what he termed “plasma-weakness” had been produced, so that the yolky part of the egg, being no longer controlled, swelled up and crushed the more protoplasmic part. He thought that the weakness was immediately due to the cutting off of oxygen by the reagent. He considered that the results which he had obtained with Amphibian eggs explained the production of the “fancy” races of goldfish, because the Chinese breeders, with whom these races originated, were accustomed to rear the spawn of their fish in foul water.

The development of rana temporaria under the influence of cane sugar solution