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In an earlier paper of this series it was shown that cholesterol is not excreted in the faces of herbivorous animals, and that when rabbits are fed on a diet free from phytosterol but containing measured quantities of cholesterol, a portion of the latter substance which is strictly conserved in the animal economy; that when the destruction of the red blood corpuscles and possibly other cells takes place in the liver, their cholesterol is excreted in the bile, and that the cholesterol of the bile is reabsorbed in the intestine along with the bile salt, and finds its way into the blood stream to be used in cell-anabolism. It was also suggested that any waste of cholesterol might possibly be made up from that taken in with the food. In order to test this view, comparative estimations were made of the total cholesterol content previously been thoroughly extracted with ether, and on the same extracted bran with the addition of known amounts of cholesterol, care being taken that the animals were otherwise identically treated and were kept in good health. The results of the experiments showed that some, at any rate, of the cholesterol absorbed found its way into the blood stream. It seemed to us blood stream as such or in the from of esters or in both states, and also whether the phytosterol of vegetable food can be utilised for the formation of cholesterol in the organism. Owing to the small percentage of these substances in the blood, it did not seem probable that the chemical methods hitherto available for their estimation were sufficiently accurate to give reliable information unless in each experiment a large number of animals was taken than we could conveniently attend to. It seemed likely, however, that a comparative study of the inhibitory effects of the sera of rabbits fed on different diets on the hæmolytic action of saponin on blood might throw light on the points mentioned.

proceedings of the royal society of london. series b, containing papers of a biological character

The origin and destiny of cholesterol in the animal organism. Part V. -On the inhibitory action of the sera of rabbits fed on diets containing varying amounts of cholesterol on the hœmolysis of blood by saponin