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In recent years, noninvasive diagnosis based on biomarkers in exhaled breath has been extensively studied. The procedure of biomarker collection is a key step. However, the traditional condenser method has low efficacy in collecting nonvolatile compounds especially the protein biomarkers in breath. To solve this deficiency, here we propose an electret filter method.Exhaled breath of 6 volunteers was collected with a glass condenser and an electret filter. The amount of albumin was analyzed. Furthermore, the difference of exhaled albumin between smokers and nonsmokers was evaluated.The electret filter method collected more albumin than the glass condenser method at the same breath volume level (P < .01). Smokers exhaling more albumin than nonsmokers were also observed (P < .01).The electret filter is capable of collecting proteins more effectively than the condenser method. In addition, smokers tend to exhale more albumin than nonsmokers.

Electret filter collects more exhaled albumin than glass condenser