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In vitro study to develop an intervertebral disc degeneration organ culture model, using coccygeal bovine intervertebral discs (IVDs) and injection of proteolytic enzymes MMP-3, ADAMTS-4, and HTRA1.

Assessment of the Matrix Degenerative Effects of MMP-3, ADAMTS-4, and HTRA1, Injected Into a Bovine Intervertebral Disc Organ Culture Model