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BACKGROUND: Adipose stromal vascular fraction (SVF) is an important source of adipose-derived mesenchymal stem cells (ADMSCs) that are used in regenerative medicine.1 Numerous procedures including mechanical and enzymatic methods have been used to obtain stromal vascular cells (SVCs) from adipose tissue.2 The cellular composition of SVF obtained may have an effect on its capabilities. Therefore, we compared the yield and cellular composition of SVCs obtained from lipoaspirates after centrifugation, vortexing and collagenase digestion.

Comparison between Stromal Vascular Cells’ Isolation with Enzymatic Digestion and Mechanical Processing of Aspirated Adipose Tissue