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(p<0.01) at 1, 4, 7, 15, and 30 dpi respectively. A cardiomyocyte proliferation index generated by MEF2/PCNA staining confirmed cardiomyocyte-specific suppression in ARF:ARF heart regeneration by 46.6% (p=0.01) at 11 dpi. Tissue-specific regenerative gene expression was tracked by qPCR in ARF:ARF and WT fish. Fgf17b, vegfaa, and Twist1b were reduced by 42% (p<0.01), 43% (p<0.01), and 55% in ARF:ARF hearts at 11 dpi, reflective of decreases in myocardial regeneration, vascular regeneration, and epithelial-to-mesenchymal (EMT) transition respectively. There was no significant difference in fgfr2c expression (p=0.44), a marker of epicardial regeneration.

Abstract 19: Extended Graft Survival After Cryopreservation and Storage Below -130°C in a Rat Orthotopic Hind Limb Model