Open AccessAbstract 19: Extended Graft Survival After Cryopreservation and Storage Below -130°C in a Rat Orthotopic Hind Limb Model
Author(s) -
Samuel A. J. Fidder,
Byoungchol Oh,
Franka Messner,
Georg Furtmueller,
Kristi L. Helke,
Elizabeth D. Greene,
Zhen Chen,
Lia H. Campbell,
Gerald Brandacher,
Kelvin G.M. Brockbank
Publication year - 2019
Publication title -
plastic and reconstructive surgery global open
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.759
H-Index - 28
ISSN - 2169-7574
DOI - 10.1097/01.gox.0000558293.28005.fc
Subject(s) - cryopreservation , medicine , hindlimb , transplantation , surgery , h&e stain , allotransplantation , cryoprotectant , anatomy , pathology , staining , biology , microbiology and biotechnology , embryo
(p<0.01) at 1, 4, 7, 15, and 30 dpi respectively. A cardiomyocyte proliferation index generated by MEF2/PCNA staining confirmed cardiomyocyte-specific suppression in ARF:ARF heart regeneration by 46.6% (p=0.01) at 11 dpi. Tissue-specific regenerative gene expression was tracked by qPCR in ARF:ARF and WT fish. Fgf17b, vegfaa, and Twist1b were reduced by 42% (p<0.01), 43% (p<0.01), and 55% in ARF:ARF hearts at 11 dpi, reflective of decreases in myocardial regeneration, vascular regeneration, and epithelial-to-mesenchymal (EMT) transition respectively. There was no significant difference in fgfr2c expression (p=0.44), a marker of epicardial regeneration.
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