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PURPOSE: A serum-free, ex vivo cell expansion system called the mononuclear cell quality and quantity control culture (MNC-QQc) system can increase the number of CD34-positive cells, which are an indicator for endothelial progenitor cells (EPCs). MNC-QQc cells have angiogenic potential that is 30 times higher than that of MNCs. Although MNC-QQc is already an effective therapy, we investigated whether microgravity (MG) can increase the potential of this culture system. MG was reported to increase the stem cell culture functionality. This study aimed to evaluate the effect of MG on MNC-QQc to increase the number and improve the function of angiogenic cells, such as EPCs.

plastic and reconstructive surgery. global open

Abstract QS17: Ex vivo Angiogenic Cell Expansion System Increases The Number and Vasculogenic Potential of Endothelial Progenitor Cells by Switching the Culture Gravity Condition