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Sarday, M ay 9, 2018 known role of these cells in homeostasis. Importantly, Treg depletion also led to a 1.4-fold increase in bacterial clearance (P=0.042) and a 4.5-fold increase in antibody production (P=0.0089) as compared to ALND-treated mice with intact Tregs. In addition, compared to Treg-intact mice, Treg-depleted mice had significantly more activated dendritic cells (DCs) both in the ipsilateral forelimb (P<0.0001) and cervical lymph nodes (the closest drainage lymph node basin after ALND) (P<0.0001) following ALND. LNT resulted in a similar mitigation of local immunosuppression. Furthermore, analysis of the transplanted axillary lymph nodes demonstrated that these lymph nodes actively participated in bacterial clearance and DC trafficking.

Abstract 122: Lymphatic Endothelial Cell Tubule Formation in 3D Culture is Inhibited by Increased Matrix Pressure