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CONCLUSIONS: Our data suggest that fibroblast phenotype is highly cell intrinsic and based on the accumulation of epigenetic change. Epigenetic change correlates with the transition in healing phenotype, and localizes to promoter sequences. By using the CRISPR-Cas9 system in future experiments, we will delineate which genes associated with e18 open promoters are the master regulators of fibrosis. Intervention at these genes may allow for scarless healing in adults.

Abstract 35: Integrating Tissue Engineering Principles for Skeletal Regeneration of the Radius and Mandible in Translational Models