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RESULTS: We successfully isolated and cultured LGR6+ ESCs from GFP expressing SD rats. We confirmed their multipotency via separate inductions towards osteoblast, chondrocyte and adipocyte lineages visible under light microscopy. The adipo-, osteoand chondro-inductions were observed at days 12, 26 and 29 respectively. Uninduced and Induced cells were shown on confocal microscopy and SEM to grow, adhere, survive and maintain their respective non-stressed or osteogenic morphology on the surface of TCPS and CCS at days 7, 14, 21, and 28. The MTT assay demonstrated that cells seeded on CCS and TCPS have no significant variation in proliferation rate between coverslip materials for either the induced or uninduced conditions.

Abstract 13: DNA Damage Signaling and Cell Senescence in BRCA1 Mutated Adipose Stem Cells Leads to Breast Cancer Progression