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M oday, O cber 9, 2017 is hindered in part by the availability of limbs which can survive a limited amount of time disconnected from the body. Cryobiology is the scientific field which investigates biology at low temperatures. Cryobiologists have long sought to cryopreserve biological samples ranging from single cells to complex organs and whole animals. Vitrification is the transformation of a substance into a non-crystalline amorphous solid using rapid heat removal. Directional freezing is a technique, consisting of highly controlled and cell-friendly ice crystal morphology that significantly reduces mechanical damage. Extremely efficient heat removal and controlled ice crystal propagation make it suitable for freezing both small and large volumes alike. Combining Directional freezing and VCA may open the door for “Organ Banking”. If composite tissue could be frozen and then thawed without damage, non transplanted tissue and organ waste would be reduced, potentially enabling better donor and recipient availability and match.

Abstract: Extended Normothermic Ex-Situ Limb Perfusion Preserves Limbs Viable and Functional Up to 44 Hours