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University of Wisconsin (UW) solution for 3 hours prior to transplant. Experimental group (n=24) flaps were perfused with MP/HBOC for 17 hours at a subnormothermic temperature of 21°C. Flaps were monitored daily for clinical evidence of viability and biopsied per protocol with an end point of 17 hours for ex vivo only, 14 days for autotransplants and 60 days for allotransplants. The allotransplanted animals were placed on systemic triple immune suppression and maintained at therapeutic levels for the duration of the study. Histologic analysis was blinded and reviewed by an expert veterinarian pathologist at conclusion of the study.

Abstract: Directional Freezing and Vitrification of Whole Limbs for Future Transplantation and Organ Banking