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Taurolidine was successfully loaded at 2,6 and 10% by weight throughout the matrix of the fibers. 400 ul of early phase Pseudomonas aeruginosa (PAO1), the Staphylococcus epidermidis (S.epi 35984), and the multidrug resistant Staphylococcus aureus strain SA BAA-44) were plated separately into square plates. 200 ul of each were introduced into 25cmX25cm plates. Four pieces of each fiber were individually placed in the plates. The fibers tested were tauroldine loaded at 2, 6 and 10% dispersed in poly e-caprolactone and 2, 6, and 10% dispersed in p-dioxanone. After 24 hours of exposure the zone of Inhibition surrounding each fiber sample was measured in mm. In order to demonstrate quantitative bacteria kills with living microorganisms, each of the fibers were placed in 12 well bottom culture discs that had 1 ml Tryptic Soy Buffer containing 100 ul of Early Phase Culture of each of the 3 bacteria: Pseudomonas aeruginosa (PAO1), Staphylococcus epidermidis (S.epi 35984) and multidrug resistant Stapylococcus aureus strain SA BAA-44). After 24 hours of exposure, kills were measured for each micro-organism tested using a quantitative analysis.

Abstract: Generation of a Novel Scaffold for In Vivo Polarization of Therapeutic Macrophages and Delivery of IL 10 to Improve Cutaneous Wound Healing