Open AccessDistribution of β1 Integrin Subunit in Rat Seminiferous Epithelium
Author(s) -
Fioretta Palombi,
Michele Salanova,
Guido Tarone,
Donatella Farini,
Mario Stefanini
Publication year - 1992
Publication title -
biology of reproduction
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.366
H-Index - 180
eISSN - 1529-7268
pISSN - 0006-3363
DOI - 10.1095/biolreprod47.6.1173
Subject(s) - biology , sertoli cell , immunofluorescence , integrin , epithelium , immunoprecipitation , spermatogenesis , testicle , andrology , medicine , antibody , endocrinology , microbiology and biotechnology , cell , immunology , genetics
We have studied the presence and distribution of beta 1 integrins in the seminiferous epithelium of prepubertal and adult rats. Our immunofluorescence data show that in the adult the antibody recognizes specific areas localized around the heads of elongating and maturing spermatids and above spermatogonia at stages I-VII. The following were found to be negative: a) areas adjacent to spermatogonia at stages IX-XIV and adjacent to spermatocytes and to round spermatids; b) spermiated spermatozoa. In the prepubertal rat, positive tubules are first apparent around Day 17 of age. Immunofluorescence and immunoprecipitation studies show that Sertoli cell monolayers from 3-wk-old rats express beta integrins in vitro.
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