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Taro (Colocasia esculenta [L.] Schott) is a root crop widely grown in tropical and subtropical countries (Banjaw 2017). It makes up about 80% of the aroids (family Araceae) cultivated in Bangladesh (Bhuiyan et al. 2011). Dasheen mosaic virus (DsMV) is a potyvirus transmitted by aphids to many aroids (Zettler and Hartman 1987). The vegetative propagation of taro contributes to the widespread distribution of viruses in this crop (Kazmi et al. 2015). In December 2016, virus-like symptoms including feathery chlorotic mottling and distortions in leaves were observed on taro (cultivar Panikachu) growing in Chandpur (23.2321° N, 90.6631° E) in Bangladesh. Thirteen taro samples with feathery mottling and distorted leaves and three nonsymptomatic leaves were collected and tested for potyvirus infection using universal potyvirus-specific ELISA (Agdia, Elkhart, IN) and universal potyvirus-specific reverse transcription polymerase chain reaction (RT-PCR) (Zheng et al. 2010), respectively. Eight of the 13 symptomatic taro samples tested positive for potyvirus in both tests, whereas the three nonsymptomatic taro leaves tested negative. To identify the specific potyvirus involved, amplicons (332 bp) of the NIb region that were generated by RT-PCR using universal potyvirus-specific primers were sequenced (accession MH036416) (Wang et al. 2017). BLASTn analysis of the sequences showed that this virus shared 87% nucleotide identity with a DsMV isolate from Florida (AF048981). Two additional sets of primers were also used to further characterize the isolate: DsMV gene CI-specific primers DMV 5708-5731-F/DMV 6131-6154-R (Wang et al. 2017); and DsMV gene CP-specific primers DMV 9398-9419-F (5′-CTTGCGTCAGATAATGCATCAC-3′)/DMV 9965-9985-R (5′-CACCGTGCACGAAGCATCTCG-3′). BLASTn analysis of the 407-bp CI amplicon (accession MH036417) shared 91% identity with a Chinese isolate of DsMV (AJ298033), and the 564-bp CP amplicon (accession MH036418) shared 92% identity with the same Chinese isolate of DsMV. Eight PCR-positive samples also tested positive for DsMV by triple-antibody sandwich ELISA using a DsMV-specific antibody (Agdia). Moreover, all 13 samples were tested for taro vein chlorosis virus (Atibalentja et al. 2018), cucumber mosaic virus (Wang et al. 2014), and taro bacilliform CH virus (Wang et al. 2017) by RT-PCR, and the results were negative. To our knowledge, this is the first report of DsMV infecting taro plants in Bangladesh. Our results will be useful for further genome characterization and management of DsMV in Bangladesh to mitigate potential threats to taro production there.

First Report of Dasheen Mosaic Virus Infecting Taro (Colocasia esculenta) in Bangladesh