Open AccessPolyubiquitin Promoter-Based Binary Vectors for Overexpression and Gene Silencing in Lotus japonicus
Author(s) -
Takaki Maekawa,
Mitsumasa Kusakabe,
Yoshikazu Shimoda,
Shusei Sato,
Satoshi Tabata,
Yoshikatsu Murooka,
Makoto Hayashi
Publication year - 2008
Publication title -
molecular plant-microbe interactions
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.565
H-Index - 153
eISSN - 1943-7706
pISSN - 0894-0282
DOI - 10.1094/mpmi-21-4-0375
Subject(s) - gene silencing , gene , lotus , genetics , binary number , lotus japonicus , biology , computational biology , botany , mathematics , mutant , arithmetic
In this study, we compared the transcriptional activities between Cauliflower mosaic virus (CaMV)35S promoter and polyubiquitin (Ljubq1) promoter from Lotus japonicus using beta-glucuronidase (gus) reporter gene in transgenic plants of L. japonicus. The promoter analysis demonstrated that the Ljubq1 promoter possessed higher activity than the CaMV35S promoter in leaves, stems, roots, nodules, and pollen. Finally, we created GATEWAY conversion technology-compatible binary vectors for over-expression and RNA interference under the Ljubq1 promoter. These materials could provide alternative choice for studies in L. japonicus.
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