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well as host/virus interaction. In our study, using 454/Illumina sequencing, we have obtained large amount of whole genome sequences. We designed a preliminary bioinformatics analysis pipeline to classify these NGS reads. First we mapped our nucleotide reads to GenBank reference sequences using BLAST, and classified them by their taxonomic family, such as host, virus and unclassified. Then, for a specific type of virus (e.g. influenza virus, MERS coronavirus), we conducted de novo and reference based assembly of the reads to obtain the full genome sequences for further phylogenetic study. In the future, through advanced bioinformatics tools, we hope to get more detailed information from our large amount of NGS sequences of field/clinical samples, experimental data, especially in the following areas: (i) finding novel pathogens in unclassified sequences; (ii) virus/virus interactions; (iii) pathogen/host interaction.

A27 Using whole genome sequence data and minority variant profiles to elucidate transmission patterns during RSV household outbreaks