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Determination of lead, cadmium, nickel, chromium, manganese and copper  levels in colostrum samples ( n  = 30), collected from  middle‐class mothers, was carried out by electrothermal atomic absorption  spectrophotometer (ETAAS) with Zeeman effect background correction using a  tungsten–palladium–citric acid chemical modifier mixture. A wet‐ashing  procedure was applied to dissolve the samples and to remove the fat. The graphite  furnace temperature programme for analytes determined by ETAAS was studied,  and the optimum pyrolysis temperatures of Pb, Cd, Ni, Cr, Mn and Cu were determined  in the presence of the modifier mixture. Detection limits of Pb, Cd, Cu, Mn  and Cr were determined using a modifier mixture solution. Zinc and iron levels  were determined by Zeeman flame atomic absorption spectrometer (F‐AAS).  Heavy metal levels (mean values) found in colostrum samples were 14.6 µg/l  of Pb, 2.8 µg/l of Cd, 27.8 µg/l of Ni, 8.6 µg/l  of Cr, 43.2 µg/l of Mn, 278 µg/l of Cu, 12.9 mg/l  of Zn, and 3.5 mg/l of Fe. These metal levels were compared with results  from other studies in different countries.

Determination of Heavy Metal Contents in Human Colostrum Samples by Electrothermal Atomic Absorption Spectrophotometry