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Myo -inositol is a vital compound in plants. As the key rate-limiting enzyme in  myo -inositol biosynthesis,  l - myo -inositol-1-phosphate synthase (MIPS) is regarded as a determinant of the  myo -inositol content in plants. The up-regulation of  MIPS  genes can increase the  myo -inositol content, thereby enhancing the plant’s resistance to a variety of stresses. However, there are few reports on the roles of  myo -inositol and the identification of  MIPS  in woody trees. In this study, a  MIPS  gene, named as  PeMIPS1 , was characterized from  Populus euphratica  Oliv. The heterologous expression of  PeMIPS1  compensated for inositol production in the yeast inositol auxotrophic mutant  ino1  and the phenotypic lesions of the  atmips1-2  mutant, an  Arabidopsis MIPS1  knock-out mutant. A subcellular location analysis showed that the PeMIPS1-GFP fusion was localized in the nucleus and cytoplasm, but not in the chloroplasts, indicating that PeMIPS1 represented the cytosolic form of MIPS in  P. euphratica . Interestingly,  PeMIPS1  was not only inducible by drought and high salinity, but also by CuSO 4  treatment. The transgenic poplar lines overexpressing  PeMIPS1  had greater plant heights, shoot biomasses and survival rates than the wild type during the salt- or copper-stress treatment, and this was accompanied by an increase in the  myo -inositol content. The overexpression of  PeMIPS1  resulted in the increased activities of antioxidant enzymes and the accumulation of ascorbate, a key nonenzymatic antioxidant in plant, which partly accounted for the enhanced reactive oxygen species-scavenging capacity and the lowered hydrogen peroxide and malondialdehyde levels in the transgenic poplar. To the best of our knowledge, this study is the first to report the roles of  MIPS  genes in the tolerance to copper stress.

Overexpression of PeMIPS1 confers tolerance to salt and copper stresses by scavenging reactive oxygen species in transgenic poplar