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Conifers exhibit a number of constitutive and induced mechanisms to defend against attack by pests and pathogens such as mountain pine beetle ( Dendroctonus ponderosae  Hopkins) and their fungal associates. Ecological studies have demonstrated that stressed trees are more susceptible to attack by mountain pine beetle than their healthy counterparts. In this study, we tested the hypothesis that water deficit affects constitutive and induced responses of mature lodgepole pine × jack pine hybrids ( Pinus contorta  Dougl. ex Loud. var.  latifolia  Engelm. ex S. Wats. ×  Pinus banksiana  Lamb.) to inoculation with the mountain pine beetle fungal associate  Grosmannia clavigera  (Robinson-Jeffrey and Davidson) Zipfel, de Beer and Wingfield. The degree of stress induced by the imposed water-deficit treatment was sufficient to reduce photosynthesis.  Grosmannia clavigera -induced lesions exhibited significantly reduced dimensions in water-deficit trees relative to well-watered trees at 5 weeks after inoculation. Treatment-associated cellular-level changes in secondary phloem were also observed. Quantitative RT-PCR was used to analyze transcript abundance profiles of 18 genes belonging to four families classically associated with biotic and abiotic stress responses: aquaporins ( AQPs ), dehydration-responsive element binding ( DREB ), terpene synthases ( TPSs ) and chitinases ( CHIs ). Transcript abundance profiles of a  TIP2 AQP  and a  TINY-like DREB  decreased significantly in fungus-inoculated trees, but not in response to water deficit. One TPS,  Pcb(+)-3-carene synthase , and the Class II CHIs  PcbCHI2.1  and  PcbCHI2.2  showed increased expression under water-deficit conditions in the absence of fungal inoculation, while another TPS,  Pcb(E)- β -farnesene synthase-like , and two CHIs,  PcbCHI1.1  and  PcbCHI4.1 , showed attenuated expression under water-deficit conditions in the presence of fungal inoculation. The effects were observed both locally and systemically. These results demonstrate that both constitutive and induced carbon- and nitrogen-based defenses are affected by water deficit, suggesting potential consequences for mountain pine beetle dynamics, particularly in novel environments.

tree physiology

Influence of water deficit on the molecular responses of  Pinus contorta  ×  Pinus banksiana  mature trees to infection by the mountain pine beetle fungal associate,  Grosmannia clavigera