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A full-length cDNA of an acyl-acyl carrier protein (ACP) thioesterase (TE) (EC 3.1.2.14), named  JcFATB1 , was isolated from the woody oil plant  Jatropha curcas  L. The deduced amino acid sequence of the cDNA shares about 78% identity with FATB TEs, but only about 33% identity with FATA TEs from other plants. The deduced sequence also contains two essential residues (H 317  and C 352 ) for TE catalytic activity and a putative chloroplast transit peptide at the N-terminal. Southern blot analysis revealed that a single copy of  JcFATB1  is present in the  J. curcas  genome, and semi-quantitative PCR analysis showed that  JcFATB1  was expressed in all tissues that were examined, most strongly in seeds, in which its expression peaked in late developmental stages. Seed-specific overexpression of the  JcFATB1  cDNA in Arabidopsis resulted in increased levels of saturated fatty acids, especially palmitate, and in reduced levels of unsaturated fatty acids. The findings suggest that  JcFATB1  from this woody oil plant can function as a saturated acyl-ACP TE and could potentially modify the seed oil of  J. curcas  to increase its levels of palmitate.

Cloning and functional characterization of an acyl-acyl carrier protein thioesterase (JcFATB1) from Jatropha curcas