English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Zendy Plus

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Zendy Tools

Zendy Open

MdCoL , which encodes a putative 2OG-Fe(II) oxygenase, is a strong candidate gene for control of the columnar growth phenotype in apple. However, the mechanism by which  MdCoL  produces the columnar trait is unclear. Here, we show that  MdCoL  influences abscisic acid (ABA) biosynthesis through its interactions with the MdDREB2 transcription factor. Expression analyses and transgenic tobacco studies have confirmed that  MdCoL  is likely a candidate for control of the columnar phenotype. Furthermore, the ABA level in columnar apple trees is significantly higher than that in standard apple trees. A protein interaction experiment has showed that  MdCoL  interacts with MdDREB2. Transient expression and electrophoretic mobility shift assays have demonstrated that MdDREB2 binds directly to the DRE motif in the  MdNCED6  and  MdNCED9  ( MdNCED6/9 ) gene promoters, thereby activating the transcription of these ABA biosynthesis genes. In addition, a higher ABA content has been detected following co-overexpression of  MdCoL –MdDREB2 when compared with the overexpression of  MdCoL  or MdDREB2 alone. Taken together, our results indicate that an interaction between  MdCoL  and MdDREB2 promotes the expression of  MdNCED6/9  and increases ABA levels, a phenomenon that may underlie the columnar growth phenotype in apple.

tree physiology

The apple columnar gene candidate  MdCoL  and the AP2/ERF factor MdDREB2 positively regulate ABA biosynthesis by activating the expression of  MdNCED6/9