English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Mutations Ser119--&gt;Glu, Ser119--&gt;Gly, Ser119--&gt;Trp, Gly121--&gt;Ala and Gly121--&gt;Ala/Ser411--&gt;Gly were constructed in glucoamylase to change substrate specificity. Mutation Ser411--&gt;Gly was already known to decrease glucoamylase selectivity toward isomaltose formation and to increase peak glucose yield. All mutated glucoamylases had slightly lower specific activities on maltose than on wild-type glucoamylase. Ser119--&gt;Glu, Ser119--&gt;Gly and Ser119--&gt;Trp glucoamylases were about as active on isomaltose and DP 4-7 maltooligosaccharides as wild-type glucoamylase. Gly121--&gt;Ala and Gly121--&gt;Ala/Ser411--&gt;Gly glucoamylases were less active. At 55 degrees C Ser119--&gt;Glu, wild-type, Ser119--&gt;Trp, Ser119--&gt;Gly, Gly121--&gt;Ala and Gly121--&gt;Ala/Ser411--&gt;Gly glucoamylases had progressively higher peak glucose yields, generally in the opposite order to their activities. There was also an inverse correlation between peak glucose yield and ratio of initial rate of isomaltose production from glucose condensation to that of glucose production from maltodextrin hydrolysis. The effect of mutations Gly121--&gt;Ala and Ser411--&gt;Gly was not additive in predicting the effect of the double mutation on the ratio or on peak glucose yield.

Mutations to alter Aspergillus awamori glucoamylase selectivity. II. Mutation of residues 119 and 121