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We determined the chlorophyll (Chi) a conversion efficiency, defined as the conversion of Chi a into pheopigments and non-fluorescent products, for the cold-water appendicularian Oiko- pleura vanhoeffeni using MGe as conservative tracer. In both laboratory experiments and in freshly collected animals, there was enough undegraded Chi a present in the gut to provide an index of feeding activity for individual animals. In laboratory experiments with diatoms, Chi a conversion efficiency was predictable with an average of 79%. The mean gut passage time (0.8 h) determined using corn starch and diatoms as markers was not influenced by food concentration or animal size over a trunk length range of 1.8-5 mm. When experimentally determined Chi a conversion efficiency and gut passage times were applied to O.vanhoeffeni from Logy Bay (Newfoundland), the estimated clearance rates were in close agreement with previously published values using several different tech- niques. We therefore suggest that the modified gut pigment technique is a useful tool to assess in situ ingestion rates of O.vanhoeffeni.

Chlorophyll a conversion and gut passage time for the pelagic tunicate Oikopleura vanhoeffeni (Appendicularia)