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• Plasmid DNA for each target was tested in duplicate at high concentration (1e6 copies/mL) for specificity of the ABRx Panel. • Linearity and sensitivity for each assay was determined using nine mixes of plasmid DNA (pDNA) over a nine-log serial dilution ranging from 100 million copies to one copy (Latin Square). • Each pDNA mix was tested in five replicates; the experiment was repeated over three days. • Data from the three experiments were compiled to determine the linear range of each assay and were also used to assess interand intra-run precision. Materials & Methods (continued)

Molecular Detection of Antimicrobial Susceptibility: Changing Paradigm of Laboratory Testing for Multidrug-Resistant Organisms