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Intraoperative simple flow cytometer (iFC) was developed in recently, we examined the correlation intraoperative frozen diagnosis (IOFD) and intraoperative frozen section of resection margin (IOFM) to iFC. METHOD AND MATERIAL Total 33 cases of high grade glioma patients were underwent operation in April 2017 ~ May 2019, and IOFD and IOFM were compared to iFC. Sample of iFC were retrieved just same parts of IOFM and IOFD. We compared to microscopical findings, MIB-1, malignant index (MI), and MI Level (G0-4). RESULTS Accuracy rate of iFC and IOFD in high grade glioma was 84%, and the concordance rate of grading was17.2%. Correlation coefficients between MIB-1 and iFC of IOFD was r=0.5019 P=0.0065. Accuracy rate of IOFM was 46.8%, and concordance rate of grading was 35.5%. Correlation coefficients between MIB-1 and iFC of IOFM was r=0.5899 P=0.0001. CONCLUSIONS Although iFC accuracy rates iFC of IOFD and IOFM were high, iFC concordance rates and grade were low. Correlation between MIB-1 and iFC of IOFM was better than that of IOFD. Probably, iFC of IOFD was a little difficult because of sample heterozygosity.

MPC-12 ACCURACY OF INTRAOPERATIVE SIMPLE FLOW CYTOMETER FOR HIGH GRADE GLIOMA OPERATION COMPARED WITH INTRAOPERATIVE FROZEN DIAGNOSIS