Open AccessRadiolytic one-electron reductive cyclization of oligodeoxynucleotides possessing a disulfide bond
Author(s) -
E. Matsumoto,
Kazuhito Tanabe,
Satoshi Nishimoto
Publication year - 2009
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/nrp089
Subject(s) - radiolysis , chemistry , aqueous solution , dna , disulfide bond , redox , combinatorial chemistry , photochemistry , organic chemistry , biochemistry
Radiolytic one-electron reduction of oligodeoxynucleotides (ODNs) possessing a disulfide bond induced strand exchange reaction in a hypoxia selective manner. In this study, we attempted to apply this radiolytic reaction to stem-and-loop structured ODNs. Hypoxic X-irradiation to an aqueous solution of stem-and-loop structured ODNs produced a cyclized DNA in good yield via an intrastrand ligation reaction.
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