Open AccessDesign of extremely facile 3'- and 5'- ends free molecular beacons using C8 alkylamino substituted 2'-deoxyguanosine
Author(s) -
Katsuhiko Matsumoto,
Yoshiaki Shinohara,
K. Numajiri,
Shinya Ishioroshi,
Takashi Morii,
Yoshio Saitō,
Isao Saito
Publication year - 2009
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/nrp071
Subject(s) - molecular beacon , pyrene , deoxyguanosine , fluorescence , chemistry , oligonucleotide , excimer , beacon , combinatorial chemistry , photochemistry , dna , organic chemistry , biochemistry , computer science , physics , quantum mechanics , real time computing
A novel fluorescent oligonucleotide probe containing pyrene-labeled C8 alkylamino-substituted 2'-deoxyguanosine and a practically useful 3'- and 5'- ends-free self-quenched molecular beacons (MB) were designed. A unique MB detectable by pyrene excimer fluorescence was also demonstrated.
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