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We analyze CD spectra of d(CGCGCGCGCGCG) to study the potential intermediate states produced during transition between both types of secondary structures of B- and Z-forms as ionic strength of PBS buffer increases. Decomposition of the CD spectra by linear superposition provides evidence for three intermediate states that distinct from B and Z-DNA. Intermediate states of B-form and Z-form found from CD spectra analysis are discussed in terms of early proposed by others the models for B- to Z-DNA transition and W-DNA model refined by molecular modelling program.

Circular dichroism spectra of d(CGCGCGCGCGCG): evidence for intermediate models in the B-to-Z transition