Open AccessSynthesis and properties of novel base-discriminating fluorescent (BDF) nucleosides
Author(s) -
Yoshio Saitō,
Kazuo Hanawa,
Keigo Hayashi,
Kaori Motegi,
Akimitsu Okaoto,
Isao Saito
Publication year - 2005
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/49.1.153
Subject(s) - fluorescence , pyrene , base (topology) , chemistry , dna , base pair , combinatorial chemistry , stereochemistry , biochemistry , organic chemistry , physics , mathematics , mathematical analysis , quantum mechanics
We designed a new type of pyrene-labeled base-discrimination fluorescent (BDF) nucleosides (Py)U, (Py)C, (8Py)A and (MePy)dA, which emitted strong fluorescence only when the bases opposite the BDF base are A, G, T and C, respectively. The DNA probes containing four different BDF bases enable us to distinguish single base alterations by simply mixing with a sample solution of target DNA.
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