Open AccessSNPs analysis by use of oligonucleotides containing 2′-O-methyl-2-thiouridines
Author(s) -
Itaru Okamoto,
Kohji Seio,
Mitsuo Sekine
Publication year - 2005
Publication title -
nucleic acids symposium series
Language(s) - English
Resource type - Journals
eISSN - 1746-8272
pISSN - 0261-3166
DOI - 10.1093/nass/49.1.123
Subject(s) - oligonucleotide , nucleotide , combinatorial chemistry , chemistry , computational biology , oligonucleotide synthesis , microbiology and biotechnology , dna , biochemistry , biology , gene
In this study, we report an improved method for the synthesis of oligonucleotides containing 2-thiouridine (s2U) derivatives. The hybridization affinity and base pair recognition ability of oligonucleotides having 2-thiouracil moieties with both the complementary RNAs and DNAs were also studied in detail. These results indicate that oligonucleotides containing s2U derivatives could be used as powerful tools for chip-based SNPs detection.
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